To efficiently prevent sample evaporation at high temperatures, we use a glass slide that is pre-poured with thermosetting oil to seal the gas-permeable PDMS. The device features 1) a suction layer that is connected to an external vacuum to generate a continuous negative pressure within the device, and 2) an outlet-free microarray layer with treelike multi-level bifurcated microchannels connecting to 4096 dead-end microwells to realize multiple loading steps until all the microwells being filled. N2 - We present herein a facile vacuum-driven microfluidic device that is capable of multi-step sample digitalization based on the gas permeability of PDMS. The authors would like to thank our funding agencies, US National Institutes of Health (grants R01AI117032, R01AI137272 and R01AI138978) for funding. If your Franklin County ancestor is among the following families, here is one book you cannot afford to do without: Akers, Bernard, Boone, Booth, Bowman, Brodie, Brown, Cahill, Callaway, Carper, Claiborne, Cooper, Craghead, Davis, Dillard, Dillon, Dudley, Early, Ferguson, Finney, Fishburn, Glass, Goode, Greer, Hancock, (Thomas) Hancock, Harper, Hill, Hook, Hopkins, (Charles) Hopkins, James, Jamison, Laprade, Lavinder, Lee, McNiel, Marshall, Martin, Mitchell, Montgomery, Motley-Martin, Naff (Naeff, Knaff), Nelson, Peters, Pinkard, Powell-Payne, Price, Prillaman, Prunty, Ross, Saunders, Swanson-Muse, Taliaferro, Tate, Tinsley, Turner, Walker, Webster, and Wingfield.T1 - A Vacuum-Driven Microfluidic Array for Multi-Step Sample Digitalization
Now that the original edition of the Wingfield work is out of print, Clearfield Company has arranged to reprint it by special courtesy of the Virginia Book Company.
Although his manuscript on the pioneer families of Franklin County-with references to nearly 15,000 persons-was completed in 1939, it remained unpublished until 1964, when the Virginia Book Company of Berryville, Virginia, issued it with the consent of Dr.
Wingfield was widely regarded as the foremost authority on the history and genealogy of Franklin County, Virginia.
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